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E. coli Recombinant β-Glucuronidase

SKU – Pack Size Price ($USD)
EBG-1ML Get price
EBG-5ML Get price

EBG, a recombinant Beta Glucuronidase from E. coli, is a highly specific enzyme used to hydrolyze glucuronides obtained from biological fluids detecting a wide range of drugs such as opioids, benzodiazepines, steroids, cannabinoids, and others. Because of its high rate of hydrolytic activity, reactions can be performed in 15-30min. Prior to hydrolysis, samples may be analyzed by enzyme immunoassay, mass spectrometry, high performance liquid chromatography, and other means.

  • – Enzymatic hydrolysis of β-glucuronides.
  • – Deconjugation of human and animal steroids
Liquid
β -Glucuronidase Activity >50,000 U/ml (Phenolphthalein-β-D-glucuronide as substrate)
>140 U/ml (Nitrophenyl-β-D-glucuronide as substrate)
Form Clear liquid solution
Storage Temperature -20 °C

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2. Beck, O., et. al., J. Anal. Toxicol., 21, 554-557 (1997).
3. Meatherall, R., and Fraser, A. J. Anal. Toxicol., 22, 270-273 (1998).
4. Klette, K., et. al. J. Anal. Toxicol., 29, 193-200 (2005).